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Fenbendazole as a Potential Anticancer Drug

cells. EMT6, a cloned, cell culture-adapted mouse mammary tumor cell line, was used in all investigations. In our earlier articles, we have characterized the genesis and features of EMT6 cells in vitro and EMT6 tumors in vivo, as well as how the cells and tumors react to radiation and other anticancer medications.

cell culture research. In Petri dishes or glass culture flasks containing Weymouth's medium supplemented with 15% serum (FetalPlexTM) and antibiotics (both from Invitrogen, Carlsbad, CA, USA), EMT6 cells were plated after being extracted from exponentially developing monolayers. The cultures were in the middle of exponential growth when they were treated and had been incubated at 37 °C in a humid environment of 95% air and 5% CO2 for three days.

By contrasting the clonogenicity of treated and untreated control cells that were plated on the same day, the survival fractions were estimated. Cell viability was also evaluated using yield-corrected surviving fractions, which take into account both the difference in cell number and the difference in clonogenicity in Fenbendazole, if the numbers of cells in the treated and control cultures were different. Data from numerous independent experiments are represented as geometric means and SEM in the graphs. Since the surviving fractions in these experiments ranged widely (from 1.0 to 0.02), it is necessary to plot the survival curves using logarithmic Y axes to enable rigorous comparison of the data over the full range of the observed survivals. This is because some of the treatment agents used in these experiments are quite effective at killing tumor cells.

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